Publications
See Pubmed for a recent list of publications.
2024
Nguyen QH, Lai CHR, Norris MJ, Ng D, Shah M, Lai CC, Isenman DE, Moraes TF. A surface lipoprotein on Pasteurella multocida binds complement factor I to promote immune evasion. bioRxiv [Preprint]. 2024 Oct 21:2024.10.21.619360. doi: 10.1101/2024.10.21.619360. PMID: 39484374
Islam EA, Fegan JE, Zeppa JJ, Ahn SK, Ng D, Currie EG, Lam J, Moraes TF, Gray-Owen SD. Adjuvant-dependent impacts on vaccine-induced humoral responses and protection in preclinical models of nasal and genital colonization by pathogenic Neisseria . bioRxiv [Preprint]. 2024 Sep 12:2024.09.07.611809. doi: 10.1101/2024.09.07.611809. PMID: 39314293
Fegan JE, Islam EA, Curran DM, Ng D, Au N, Currie EG, Zeppa J, Lam J, Schryvers AB, Moraes TF*, Gray-Owen SD*. Rational selection of TbpB variants elucidates a bivalent vaccine formulation with broad spectrum coverage against Neisseria gonorrhoeae. bioRxiv [Preprint]. 2024 Sep 7:2024.09.07.611798. doi: 10.1101/2024.09.07.611798.PMID: 39282273
Curran DM, Ng D, Parkinson J, Moraes TF, Gray-Owen S, Fegan FE. Phylogeny-based selection of representative variants with Navargator: Proof of principle using humoral cross-reactivity data from two immunization studies. bioRxiv, 2024.09. 08.611914
Mellouk A, Jaouen P, Ruel LJ, Lê M, Martini C, Moraes TF, El Bakkouri M, Lagüe P, Boisselier E, Calmettes C. POTRA domains of the TamA insertase interact with the outer membrane and modulate membrane properties.Proc Natl Acad Sci U S A. 2024 Jul 9;121(28):e2402543121. doi: 10.1073/pnas.2402543121. Epub 2024 Jul 3.PMID: 38959031
Shah R, Panagiotou TC, Cole GB, Moraes TF, Lavoie BD, McCulloch CA, Wilde A. The DIAPH3 linker specifies a β-actin network that maintains RhoA and Myosin-II at the cytokinetic furrow. Nat Commun. 2024 Jun 19;15(1):5250. doi: 10.1038/s41467-024-49427-2. PMID: 38897998
Awate OA, Ng D, Stoudenmire JL, Moraes TF, Cornelissen CN. Investigating the importance of selected surface-exposed loops in HpuB for hemoglobin binding and utilization by Neisseria gonorrhoeae Infect Immun. 2024 Jun 12:e0021124. doi: 10.1128/iai.00211-24. Online ahead of print. PMID: 38864605
Shin HE, Pan C, Curran DM, Bateman TJ, Chong DHY, Ng D, Shah M, Moraes TF. Prevalence of Slam-dependent hemophilins in Gram-negative bacteria. J Bacteriol. 2024 May 30:e0002724. doi: 10.1128/jb.00027-24. Online ahead of print. PMID: 38814789
Fegan JE, Waeckerlin RC, Tesfaw L, Islam EA, Deresse G, Dufera D, Assefa E, Woldemedhin W, Legesse A, Akalu M, Bayissa B, Nguyen QH, Ng D, Ahn SK, Schryvers AB, Tefera TA, Moraes TF*, Gray-Owen SD*. Developing a PmSLP3-based vaccine formulation that provides robust long-lasting protection against hemorrhagic septicemia-causing serogroup B and E strains of Pasteurella multocida in cattle . Front Immunol. 2024 May 21;15:1392681. doi: 10.3389/fimmu.2024.1392681. eCollection 2024.PMID: 38835751
Ostan NKH, Cole GB, Wang FZ, Reichheld SE, Moore G, Pan C, Yu R, Lai CC, Sharpe S, Lee HO, Schryvers AB, Moraes TF. A secreted bacterial protein protects bacteria from cationic antimicrobial peptides by entrapment in phase-separated droplets. PNAS Nexus. 2024 Apr 2;3(4):pgae139. doi: 10.1093/pnasnexus/pgae139. eCollection 2024 Apr. PMID: 38633880
Huynh MS, Xu JC, Moraes TF. Examining Protein Translocation by β-Barrel Membrane Proteins Using Reconstituted Proteoliposomes Methods Mol Biol. 2024;2778:83-99. doi: 10.1007/978-1-0716-3734-0_6. PMID: 38478273
Maunders EA, Giles MW, Ganio K, Cunningham BA, Bennett-Wood V, Cole GB, Ng D, Lai CC, Neville SL, Moraes TF, McDevitt CA, Tan A. Zinc acquisition and its contribution to Klebsiella pneumoniae virulence . Front Cell Infect Microbiol. 2024 Jan 5;13:1322973. doi: 10.3389/fcimb.2023.1322973. eCollection 2023. PMID: 38249299
2023
Ferguson MR, Delgado KN, McBride S, Orbe IC, La Vake CJ, Caimano MJ, Mendez Q, Moraes TF, Schryvers AB, Moody MA, Radolf JD, Weiner MP, Hawley KL. Use of Epivolve phage display to generate a monoclonal antibody with opsonic activity directed against a subdominant epitope on extracellular loop 4 of Treponema pallidum BamA (TP0326) . Front Immunol. 2023 Aug 22;14:1222267. doi: 10.3389/fimmu.2023.1222267. eCollection 2023. PMID: 37675118
Ng D, Moraes TF. Dynamic players and intricate interactions: An integrated investigation of the Mla lipid transport system. J Biol Chem. 2023 Sep;299(9):105146. doi: 10.1016/j.jbc.2023.105146. Epub 2023 Aug 9. PMID: 37562569
Kejiou NS, Ilan L, Aigner S, Luo E, Tonn T, Ozadam H, Lee M, Cole GB, Rabano I, Rajakulendran N, Yee BA, Najafabadi HS, Moraes TF, Angers S, Yeo GW, Cenik C, Palazzo AF. Pyruvate Kinase M (PKM) binds ribosomes in a poly-ADP ribosylation dependent manner to induce translational stalling. Nucleic Acids Res. 2023 Jul 7;51(12):6461-6478. doi: 10.1093/nar/gkad440. PMID: 37224531
Hwang S, Shah M, Garcia B, Hashem N, Davidson AR, Moraes TF, Maxwell KL. Anti-CRISPR Protein AcrIIC5 Inhibits CRISPR-Cas9 by Occupying the Target DNA Binding Pocket. J Mol Biol. 2023 Apr 1;435(7):167991. doi: 10.1016/j.jmb.2023.167991. Epub 2023 Feb 2.PMID: 36736884
Islam EA, Fegan JE, Tefera TA, Curran DM, Waeckerlin RC, Ng D, Ahn SK, Lai CHR, Nguyen QH, Shah M, Tesfaw L, Adamu K, Medhin WW, Legesse A, Deresse G, Getachew B, Rawlyk N, Evans B, Potter A, Schryvers AB*, Gray-Owen SD*, Moraes TF*. Reverse vaccinology-based identification of a novel surface lipoprotein that is an effective vaccine antigen against bovine infections caused by Pasteurella multocida. PLoS Pathog. 2023 Mar 24;19(3):e1011249. doi: 10.1371/journal.ppat.1011249. eCollection 2023 Mar.PMID: 36961851
2022
Huynh MS, Hooda Y, Li YR, Jagielnicki M, Lai CC, Moraes TF. Reconstitution of surface lipoprotein translocation through the slam translocon. Elife. 2022 Apr 27;11:e72822. doi: 10.7554/eLife.72822. Online ahead of print.PMID: 35475756
2021
Hwang S, Pan C, Garcia B, Davidson AR, Moraes TF, Maxwell KL. Structural and Mechanistic Insight into CRISPR-Cas9 Inhibition by Anti-CRISPR Protein AcrIIC4Hpa.J Mol Biol. 2022 Mar 15;434(5):167420. doi: 10.1016/j.jmb.2021.167420. Epub 2021 Dec 23.PMID: 34954237
Bateman TJ, Shah M, Ho TP, Shin HE, Pan C,Harris G, Fegan JE, Islam EA,Ahn SK, Hooda Y, Gray-Owen SD, Chen W, Moraes TF.A Slam-dependent hemophore contributes to heme acquisition in the bacterial pathogen Acinetobacter baumannii.Nature Communications. 2021 Nov 1;12(1):6270. doi: 10.1038/s41467-021-26545-9.PMID: 34725337
Pan C, Zimmer A, Shah M, Huynh MS, Chieh-Lin Lai C, Sit B, Hooda Y, Curran DM, Moraes TF. Actinobacillus utilizes a binding protein-dependent ABC transporter to acquire the active form of vitamin B6. Journal of Biological Chemistry. 2021 Aug 3:101046. doi: 10.1016/j.jbc.2021.101046. PMID: 34358566
Chen A, Ulloa Severino L, Panagiotou TC, Moraes TF, Yuen DA, Lavoie BD, Wilde A . Inhibition of polar actin assembly by astral microtubules is required for cytokinesis. Nature Communications. 2021 Apr 23;12(1):2409. doi: 10.1038/s41467-021-22677-0. PMID: 33893302
Shah M,Taylor VL, Bona D, Tsao Y, Stanley SY, Pimentel-Elardo SM, McCallum M, Bondy-Denomy J, Howell PL, Nodwell JR, Davidson AR, Moraes TF, Maxwell KL. A phage-encoded anti-activator inhibits quorum sensing in Pseudomonas aeruginosa.Molecular Cell. 2021 Jan 4:S1097-2765(20)30903-5. doi: 10.1016/j.molcel.2020.12.011.
Ostan NKH, Moraes TF , Schryvers AB. Lactoferrin receptors in Gram-negative bacteria: an evolutionary perspective. Biochem Cell Biol . 2021 Jan 19:1-7. doi: 10.1139/bcb-2020-0079.
Cole GB, Bateman TJ, Moraes TF. The surface lipoproteins of gram-negative bacteria: Protectors and foragers in harsh environments. J Biol Chem. 2021 Jan-Jun;296:100147. 2021 Jan;296:100147. doi: 10.1074/jbc.REV120.008745. Epub 2020 Dec 10. PMID: 33277359.
2020
Wu Z, Andersen PL, Moraes T, McKenna SA, Zhang Y, Zhang W, Ellison MJ, Xiao W. Uev1A amino terminus stimulates poly-ubiquitin chain assembly and is required for NF-kappaB activation.Cell Signal. 2020 Oct;74:109712. doi: 10.1016/j.cellsig.2020.109712.
AK Pogoutse, TF Moraes Transferrin binding protein B and Transferrin binding protein A 2 expand the transferrin recognition range of Histophilus somni.Journal of Bacteriology 2020 Jun 25;202(14):e00177-20. doi: 10.1128/JB.00177-20. Print 2020 Jun 25.PMID: 32366593
Chen A, Arora PD, Lai CC, Copeland JW, Moraes TF, McCulloch CA, Lavoie BD, Wilde A. The scaffold-protein IQGAP1 enhances and spatially restricts the actin-nucleating activity of Diaphanous-related formin 1 (DIAPH1). J Biol Chem. 2020 Mar 6;295(10):3134-3147. doi: 10.1074/jbc.RA119.010476. Epub 2020 Jan 31.
Yadav R, Noinaj N, Ostan N, Moraes T, Stoudenmire J, Maurakis S, Cornelissen CN.Structural Basis for Evasion of Nutritional Immunity by the Pathogenic Neisseriae. Front Microbiol. 2020 Jan 10;10:2981. doi: 10.3389/fmicb.2019.02981. eCollection 2019. Review.
2019
Thavalingam A, Cheng Z, Garcia B, Huang X, Shah M, Sun W, Wang M, Harrington L, Hwang S, Hidalgo-Reyes Y, Sontheimer EJ, Doudna J, Davidson AR, Moraes TF, Wang Y, Maxwell KL.Inhibition of CRISPR-Cas9 ribonucleoprotein complex assembly by anti-CRISPR AcrIIC2. Nature Communications. 2019 Jun 26;10(1):2806. doi: 10.1038/s41467-019-10577-3.
Fegan JE, Calmettes C, Islam EA, Ahn SK, Chaudhuri S, Yu RH, Gray-Owen SD, Moraes TF, Schryvers AB. Utility of Hybrid Transferrin Binding Protein Antigens for Protection Against Pathogenic Neisseria Species. Frontiers Immunology 2019 Feb 19;10:247. doi: 10.3389/fimmu.2019.00247. eCollection 2019.
2018
Bai X, Moraes TF,Reithmeier RAF. Structural biology of solute carrier (SLC) membrane transport proteins.Mol Membr Biol. 2018 Apr 13:1-32. doi: 10.1080/09687688.2018.1448123.
Hooda Y, Moraes TF. Translocation of lipoproteins to the surface of gram negative bacteria. Curr Opin Struct Biol. 2018 Mar 23;51:73-79. doi: 10.1016/j.sbi.2018.03.006. Review.
*Babu M, Bundalovic-Torma C, Calmettes C, Phanse S, Zhang Q, Jiang Y, Minic Z, Kim S, Mehla J, Gagarinova A, Rodionova I, Kumar A, Guo H, Kagan O, Pogoutse O, Aoki H, Deineko V, Caufield JH, Holtzapple E, Zhang Z, Vastermark A, Pandya Y, Lai CC, El Bakkouri M, Hooda Y, Shah M, Burnside D, Hooshyar M, Vlasblom J, Rajagopala SV, Golshani A, Wuchty S, F Greenblatt J, *Saier M, *Uetz P, *Moraes TF, *Parkinson J, *Emili A. Global landscape of cell envelope protein complexes in Escherichia coli. Nature Biotechnology 2018 Jan;36(1):103-112. doi: 10.1038/nbt.4024.
2017
Pawluk A, Shah M, Mejdani M, Calmettes C, Moraes TF, Davidson AR, Maxwell KL. Disabling a Type I-E CRISPR-Cas Nuclease with a Bacteriophage-Encoded Anti-CRISPR Protein. MBio.2017 Dec 12;8(6). pii: e01751-17. doi: 10.1128/mBio.01751-17.
Hooda Y, Lai CCL, Moraes TF. Identification of a Large Family of Slam-Dependent Surface Lipoproteins in Gram-Negative Bacteria. Front Cell Infect Microbiol. 2017 May 31;7:207. doi: 10.3389/fcimb.2017.00207
Hooda Y, Shin HE, Bateman TJ, Moraes TF. Neisserial surface lipoproteins: structure, function and biogenesis. Pathog Dis. 2017 Mar 1;75(2). doi: 10.1093/femspd/ftx010.
Pogoutse AK, Moraes TF. Iron acquisition through the bacterial transferrin receptor. Crit Rev Biochem Mol Biol. 2017 Jun;52(3):314-326. doi: 10.1080/10409238.2017.1293606.
Ostan NK, Yu RH, Ng D, Lai CC, Pogoutse AK, Sarpe V, Hepburn M, Sheff J, Raval S, Schriemer DC, Moraes TF, Schryvers AB. Lactoferrin binding protein B - a bi-functional bacterial receptor protein. PLoS Pathog. 2017 Mar 3;13(3):e1006244. doi: 10.1371/journal.ppat.1006244.
2016
Almawi AW, Matthews LA, Larasati, Myrox P, Boulton S, Lai C, Moraes T, Melacini G, Ghirlando R, Duncker BP, Guarné A. 'AND' logic gates at work: Crystal structure of Rad53 bound to Dbf4 and Cdc7. Sci Rep. 2016 Sep 29;6:34237. doi: 10.1038/srep34237.
McCallum M, Tammam S, Little DJ, Robinson H, Koo J, Shah M, Calmettes C, Moraes TF, Burrows LL, Howell PL. PilN Binding Modulates the Structure and Binding Partners of the Pseudomonas aeruginosa Type IVa Pilus Protein PilM. J Biol Chem. 2016 May 20;291(21):11003-15. doi: 10.1074/jbc.M116.718353.
Thurlow B, Davis JH, Leong VF, Moraes T, Williamson JR, Ortega J. Binding properties of YjeQ (RsgA), RbfA, RimM and Era to assembly intermediates of the 30S subunit. Nucleic Acids Res. 2016 Nov 16;44(20):9918-9932.
Y. Hooda, C.C. Lai, A. Judd, C. Buckwalter, H.E. Shin, S.D. Gray-Owen, and T.F. Moraes. Slam is an outer membrane protein that is required for the surface display of lipidated virulence factors in Neisseria. Nature Microbiology. 2016 Feb 29;1:16009. doi: 10.1038/nmicrobiol.2016.9.
Anastassia K. Pogoutse, Christine C. Lai, Nick Ostan, Rong-hua Yu, Anthony B. Schryvers, Trevor F. Moraes. A method for measuring binding constants using unpurified in vivo biotinylated ligands. Analytical Biochemistry. 2016 May 15;501:35-43. doi: 10.1016/j.ab.2016.02.001. Epub 2016 Feb 18.
Xiaoyun Bai,Trevor F. Moraes and Reinhart R.A. Reithmeier. Effect of SLC26 Anion Transporter Disease-causing Mutations on the Stability of the Homologous STAS Domain of E. coli DauA (YchM).Biochem J. 2015 Dec 3. pii: BJ20151025.
2015
Charles Calmettes, Andrew Judd, Trevor F. Moraes. Structural Aspects of Bacterial Outer Membrane Protein Assembly. Adv Exp Med Biol. 2015;883:255-70. doi: 10.1007/978-3-319-23603-2_14.
Reinhart R.A. Reithmeier, Trevor F. Moraes. Solute carriers keep on rockin'. Nat Struct Mol Biol. 2015 Oct 6;22(10):752-4. doi: 10.1038/nsmb.3104.
Brandon Sit, Shauna M. Crowley, Kirandeep Bhullar, Christine Chieh-Lin Lai, Calvin Tang, Yogesh Hooda, Charles Calmettes, Husain Khambati, Caixia Ma, John H. Brumell, Anthony B. Schryvers, Bruce A. Vallance, and Trevor F. Moraes. Active Transport of Phosphorylated Carbohydrates Promotes Intestinal Colonization and Transmission of a Bacterial Pathogen. PLoS Pathog. 2015 Aug 21;11(8):e1005107. doi: 10.1371/journal.ppat.1005107
Charles Calmettes, Christopher Ing, Carolyn M. Buckwalter, Majida El Bakkouri, Christine Chieh-Lin Lai, Pogoutse A, Scott D Gray-Owen, Régis Pomès and Trevor F. Moraes. The molecular mechanism of Zinc acquisition by the neisserial outer-membrane transporter ZnuD. Nat Commun. 2015 Aug 18;6:7996. doi: 10.1038/ncomms8996.
Paul Adamiak, Charles Calmettes, Trevor F. Moraes, and Anthony B. Schryvers. Patterns of structural and sequence variation within isotype lineages of the Neisseria meningitidis transferrin receptor system. MicrobiologyOpen. 2015 Jun;4(3):491-504. doi: 10.1002/mbo3.254.
Frandoloso R, Martínez-Martínez S,Calmettes C, Fegan J, Costa E, Curran D, Yu R, Gutiérrez-Martín CB, Rodríguez Ferri EF, Moraes TF, Schryvers AB. Nonbinding site-directed mutants of transferrin binding protein B exhibit enhanced immunogenicity and protective capabilities. Infect Immun. 2015 Mar;83(3):1030-8
2013
Morgenthau A, Pogoutse A, Adamiak P, Moraes TF, Schryvers AB. (2013) Bacterial receptors for host transferrin and lactoferrin: molecular mechanisms and role in host-microbe interactions. Future Microbiol. 8(12): 1575-85
Shnitsar V, Li J, Li X, Calmettes C, Basu A, Casey JR, Moraes TF, Reithmeier RA. (2013) A substrate access tunnel in the cytosolic domain is not an essential feature of the solute carrier 4 (SLC4) family of bicarbonate transporters. J Biol Chem. doi:10.1074/jbc.M113.511865.
Malott RJ, Keller BO, Gaudet RG, McCaw SE, Lai CC, Dobson-Belaire WN, Hobbs JL, St Michael F, Cox AD, Moraes TF, Gray-Owen SD. (2013) Neisseria gonorrhoeae-derived heptose elicits an innate immune response and drives HIV-1 expression. Proc Natl Acad Sci U S A. 2013 Jun 18;110(25):10234-9. doi: 10.1073/pnas.1303738110.
2012
Moraes TF, Reithmeier RA.(2012) Membrane transport metabolons. Biochim Biophys Acta. 2012 Nov;1818(11):2687-706.
Calmettes C, Alcantara J., Yu RH, Schryvers AB, Moraes T.F. (2012) The structural basis of transferrin sequestration by transferrin-binding protein B. Nat Struct Mol Biol. 2012 Feb 19;19(3):358-60. doi: 10.1038/nsmb.2251.
2011
Yang X, Yu RH, Calmettes C, Moraes T.F., and Schryvers, A.B. (2011) Anchor peptide of transferrin-binding protein B is required for interaction with transferrin-binding protein A. J. Biol. Chem. 286(52):45165-73.
Silva LP, Yu RH, Calmettes C, X. Yang, Moraes T.F., Schryvers, A.B., and Schriemer, D.C. (2011) A conserved interaction between transferrin and transferrin-binding proteins from porcine pathogens. J. Biol. Chem. 286(24):21353-60.
Calmettes C, Yu RH, Silva LP, Curran D, Schriemer DC, Schryvers AB, Moraes T.F. (2011) Structural variations within the transferrin binding site on transferrin binding protein B, TbpB. J. Biol. Chem.286(14):12683-92.
2010
Babu M, Greenblatt JF, Emili A, Strynadka NC, Reithmeier RA, Moraes T.F. (2010) Structure of a SLC26 anion transporter STAS domain in complex with acyl carrier protein: implications for E. coli YchM in fatty acid metabolism. Structure. 18(11):1450-62.
Khambati HK, Moraes T.F., Singh J, Shouldice SR, Yu RH, Schryvers AB. (2010) The role of vicinal tyrosine residues in the function of Haemophilus influenzae ferric-binding protein A. Biochem J. 432(1):57-64.
2009
Moraes T.F., Yu RH, Strynadka NC, Schryvers AB.(2009) Insights into the bacterial transferrin receptor: the structure of transferrin-binding protein B from Actinobacillus pleuropneumoniae. Molecular Cell. 35(4):523-33.
Pelzer L., Pastushok L., Moraes T., Mark Glover J.N., Ellison M.J., Ziola B., Xiao W. (2009) Biological significance of structural differences between two highly conserved Ubc variants. Biochem Biophys Res Commun. 378(3): 563-68.
Dalal, K., Nguyen, N., Alami, M., Tan, J., Moraes T.F., Sligar, S., Brayer, G., and Duong, F. (2009) Structure, binding and activity of Syd, a SecY interacting Protein.
2008
Okon M., Moraes T.F., Lario P.I., Creagh L., McIntosh L. and Strynadka N.C.J. (2008) Structural characterization of the type III pilot-secretin complex from Shigella flexneri. Structure 16(10): 1544-54.
Moraes T.F., Spreter T. and Strynadka N.C.J. (2008) Piecing together the Type III Injectisome of Bacterial Pathogens. Current Opinion in Structural Biology: Macromolecular Assemblies 18(2): 258-66.
2007
Moraes T.F., Bains M., Hancock R.E.W., Strynadka N.C.J. (2007) An arginine ladder in OprP mediates phosphate-specific transfer across the outer membrane. Nature Structural and Molecular Biology 14: 85-87. PDB id: 2o4v
2005
Ptak C., Varelas X., Moraes T., McKenna S., Ellison M.J. (2005) Purification and properties of the ubiquitin-conjugating enzymes Cdc34 and Ubc13•Mms2. Methods Enzymol. 398: 43-54.
Anderson P.L., Zhou H., Pastushok L., Moraes T.F., McKenna S., Ziola B., Ellison M.J., Xiao W. (2005) Distinct regulation of Ubc13 functions by the two ubiquitin-conjugating enzyme variants Mms2 and Uev1A. J. Cell. Biol. 170(5): 745-55.
Pastushok L., Moraes T.F., Ellison M.J., Xiao W. (2005) A single Mms2 "key" residue insertion into a Ubc13 pocket determines the interface specificity of a human Lys63 ubiquitin conjugation complex. J. Biol. Chem. 280(18): 17891-900.
2003
Mckenna S.A., Hu J., Moraes T., Xiao W., Ellison M.J., and Spyracopoulos L. (2003) Energetics and specificity of interactions within Ub•UEV•Ubc13 human ubiquitin conjugation complexes. Biochemistry 42(26): 7922-30.
Mckenna S.A., Moraes T., Xiao W., Ptak C., Spyracopoulos L., and Ellison M.J. (2003) An NMR based model of the ubiquitin-bound human ubiquitin conjugation complex Mms2/Ubc13: The structural basis for lysine 63 chain catalysis. J. Biol. Chem. 278(15): 13151-58.
2002
Rivoal J., Smith C.R., Moraes T.F., Turpin D.H., and Plaxton W.C. (2002) Method for Activity Staining after Native Polyacrylamide Gel Electrophoresis Using a Coupled Enzyme Assay and Fluorescence Detection: Application to the Analysis of Several Glycolytic Enzymes. Analytical Biochemistry 300: 94-99.
2001
McKenna S., Spyracopoulos L., Moraes T., Pastushok L., Ptak C., Xiao W. and Ellison M.J. (2001) Non-covalent interaction between ubiquitin and the DNA repair protein Mms2 is required for Ubc13-mediated poly-ubiquitination. J. Biol. Chem. 276: 40120-26.
Moraes T.F., Edwards R.A., McKenna S., Pastushok L., Xiao, W., Glover J.N.M. and Ellison M.J. (2001) Crystal structure of the human ubiquitin conjugating enzyme complex, hMms2-hUbc13. Nature Structural Biology 8: 669-73.
2000
Moraes T.F., and Plaxton W.C. (2000) Purification and characterization of phosphoenolpyruvate carboxylase from Brassica napus (oilseed rape) suspension cell cultures: Implications for phosphoenolpyruvate carboxylase regulation during phosphate starvation, and the integration of glycolysis with nitrogen assimilation. Eur. J. Biochem. 267(14): 4456-76.